An ethnomedicine study of the Tengger Tribe in Probolinggo East Java and anti-inflammatory activity
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Abstract
The Tengger Tribe in East Java, Indonesia, has long utilized medicinal plants to treat various diseases, including inflammatory conditions. This study aimed to identify medicinal plants used by the Tengger Tribe and determine their anti-inflammatory activity through in vitro and in vivo assays. An ethnomedicinal survey was conducted using snowball sampling and semi-structured interviews with key informants. Plant importance was assessed using the Use Value and Informant Consensus Factor indices. Selected plants were extracted with ethanol and evaluated for anti-inflammatory activity using the Human Red Blood Cell membrane stabilization assay in vitro and a carrageenan-induced paw edema model in vivo. Four medicinal plant species with high ethnomedicinal significance were selected for biological evaluation. Among the tested extracts, Borreria laevis exhibited the strongest anti-inflammatory activity, with an IC50 value of 133.57 ppm in the HRBC assay. The experimental data met the assumption of ANOVA p > 0.000). In the in vivo model, Tepung Otot (Borrira laevis) showed the highest anti-inflammatory effect, with inhibition percentages of 76.39%, 79.27%, and 81.55% at doses of 105, 210, and 560 mg/kg body weight. These findings support the traditional use of Tengger medicinal plants and highlight Tepung Otot (Borreria laevis) as a promising natural anti-inflammatory agent.
Article Details
Bhagawan, W. S., Ekasari, W., & Agil, M. (2023). Ethnopharmacology of medicinal plants used by the Tenggerese community in Bromo Tengger Semeru National Park, Indonesia. Biodiversitas, 24(10), 5464–5477. https://doi.org/10.13057/biodiv/d241028
Bhagawan, W. S., Ekasari, W., & Agil, M. (2025). Ethnomedicinal survey and scientific validation of inflammation-healing plants used by the Tengger community in East Java, Indonesia. Biodiversitas, 26(7), 3160–3173. https://doi.org/10.13057/biodiv/d260707
Fatima, H., Shahid, M., Jamil, A., & Naveed, M. (2021). Therapeutic Potential of Selected Medicinal Plants Against Carrageenan Induced Inflammation in Rats. Dose-Response, 19(4). https://doi.org/10.1177/15593258211058028
Febriyanti, R. M., Ramadhania, Z. M., Tjitraresmi, A., Indradi, R. B., Maisyarah, I. T., Maundu, P. M., Muhaimin, M., & Sujarwo, W. (2026). A systematic review of ethnobotanical study in Indonesia: diversity and cultural patterns of medicinal plant use. Journal of Ethnobiology and Ethnomedicine. https://doi.org/10.1186/s13002-026-00879-4
Fujiati, F., Haryati, H., Joharman, J., & Utami, S. W. (2022). In Vitro Metabolite Profiling and Anti-Inflammatory Activities of Rhodomyrtus Tomentosa with Red Blood Cell Membrane Stabilization Methods. In Reports of Biochemistry & Molecular Biology (Vol. 11, Number 3). www.RBMB.net
Jadid, N., Kurniawan, E., Himayani, C. E. S., Andriyani, Prasetyowati, I., Purwani, K. I., Muslihatin, W., Hidayati, D., & Tjahjaningrum, I. T. D. (2020). An ethnobotanical study of medicinal plants used by the Tengger tribe in Ngadisari village, Indonesia. PLoS ONE, 15(7 July). https://doi.org/10.1371/journal.pone.0235886
Lahardo, D., Ekasari, W., & Widyawaruyanti, A. (2024). Ethnomedicine Study of Medicinal Plants for Therapy of Elderly Sleep Disorders in Tengger Tribe. Borneo Journal of Pharmacy, 7(3), 306–314. https://doi.org/10.33084/bjop.v7i3.7272
Mancipe, J. C., Vargas-Pinto, P., Rodríguez, O. E., Borrego-Muñoz, P., Castellanos Londoño, I., Ramírez, D., Piñeros, L. G., Mejía, M. C., & Pombo, L. M. (2023). Anti-Inflammatory Effect of Izalpinin Derived from Chromolaena leivensis: ?-Carrageenan-Induced Paw Edema and In Silico Model. Molecules, 28(9). https://doi.org/10.3390/molecules28093722
Ningsih, I. Y., Mandasari, V. F., Pratama, A. N. W., & Hidayat, M. A. (2024). Ethnopharmacology study of medicinal plants utilization for antidiarrheal remedies by Tengger tribe in Tosari District, Indonesia. Pharmaciana, 14(1), 100. https://doi.org/10.12928/pharmaciana.v14i1.28099
Nugraha, A. S., Agustina, R. P., Mirza, S., Rani, D. M., Winarto, N. B., Triatmoko, B., Pratama, A. N. W., Keller, P. A., & Wangchuk, P. (2022). Phytochemistry and Pharmacology of Medicinal Plants Used by the Tenggerese Society in Java Island of Indonesia. In Molecules (Vol. 27, Number 21). MDPI. https://doi.org/10.3390/molecules27217532
Nugraha, A. S., Pratama, A. N. W., Triatmoko, B., Agustina, R. P., Mirza, S., Nawiyanto, Wangchuk, P., & Keller, P. A. (2024). Tenggerese traditional medicine of Indonesia: Existing practices and the botanical identification of medicinal plants. Ethnobotany Research and Applications, 27(Special Issue). https://doi.org/10.32859/era.27.39.1-21
Renny, A., Sidhic, J., Tom, A., Kuttithodi, A. M., Job, J. T., Rajagopal, R., Alfarhan, A., & Narayanankutty, A. (2024). Methanol Extract of Thottea siliquosa (Lam.) Ding Hou Leaves Inhibits Carrageenan- and Formalin-Induced Paw Edema in Mice. Molecules, 29(20). https://doi.org/10.3390/molecules29204800
Yesmin, S., Paul, A., Naz, T., Rahman, A. B. M. A., Akhter, S. F., Wahed, M. I. I., Emran, T. Bin, & Siddiqui, S. A. (2020). Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba). Clinical Phytoscience, 6(1). https://doi.org/10.1186/s40816-020-00207-7
Zhang, H., Shang, C., Tian, Z., Amin, H. K., Kassab, R. B., Abdel Moneim, A. E., & Zhang, Y. (2020). Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in Mice. Mediators of Inflammation, 2020. https://doi.org/10.1155/2020/8508906
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