Effect of Vitamin E Against Levels Malondyaldehyde (Mda) and Estrogen Hormone Levels Rats (Rattus Norvegicus) Females Who Exposed to Cigarette Smoke

  • Widya Sari Intan STIKes Hang Tuah Pekanbaru
Keywords: Vitamin E, Estrogen, Malondyaldehyde (MDA)


Indonesia is one of the countries with the greatest prevalence of smokers in the world. Exposure to cigarette smoke can lead to the physiological impact for women that can inhibit estrogen synthesis in the ovaries and increase levels of Malondyaldehyde (MDA). The purpose of this study to analyze the effect of vitamin E against MDA and estrogen rat (Rattus norvegicus) females were exposed to smoke. This research is an experimental design Post-Test Only Control Group. The total sample consisted of 30 rats females were divided into 5 groups: negative control group without treatment, the positive control group were given exposure to cigarette smoke 1 cigarettes / day and 3 treatment groups P1, P2 and P3 with vitamin E doses of multilevel: 100, 200 and 400 mg / kg / day and exposure to tobacco smoke 1 cigarettes / day. Research carried out for 21 days at Animal house and in the Biomedical Laboratory Faculty of Medicine, University of Andalas Padang. MDA and estrogen hormone levels were measured using ELISA method. Test of significance with One Way ANOVA followed by Bonferroni Multiple Comparisons types. The results showed significant differences (p <0.05) MDA levels between the negative control group (0.53 ± 0.04) positive control group (0.67 ± 0.07). In the estrogen levels also differed significantly (p <0.05) between the negative control group (0.27 ± 0.04) with P2 group (0.38 ± 0.07). In conclusion, there is no effect of vitamin E against MDA both in the negative control group and positive control group.


Pichandi, S. Pasupathi, P. Rao, YY. Farook, J., Ponnusha, BS. Ambika, A. et al. The Effect of Smoking on Canser- A review. Intarnational Journal of Biological & Medical Reasearch. 2011; 2(2): 593-602.

Halliwell, B. The Wanderings of a Free Radical. Journal of Elsevier. 2009.

Galano, A. Free Radicals Induced Oxidative Stress at a Molecular Level: The Current Status, Challenges and Perspectives of Computational Chemistry Based Protocols. Journal Mexico Chem. Soc. 2015; 59(4): 231-262.

Ayala, A. Munoz, MF. Argiieles, S. Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonanal. Oxidative Medicine and Cellular Longevity. 2014: article ID 360438: 31.

Beshay, VE. & Carr, BR. Hypothalamic-Pituitary-Ovarian Axis and Control of the Menstrual Cycle. University of Texas: 2013.

Colombo, ML. An Update on Vitamin E, Tocopherol and Tocotrienol-Perspective. Journal Molecules. 2010; 15:2103-2113.

Rizvi, S. Raza, ST. Ahmed, F. Ahmad, A. Abbas, S. Mahdi, F. The Role of Vitamin E in Human Health and Some Disease. Sultan Qaboos University Journal. 2013; 14(2):157-165.

Ryan, MJ. Dudash, HJ. Docherty, M. Geronilla, KB. Baker, BA. Haff, GG. et al. Vitamin E and C Supplementation Reduces Oxidative Stress, Improves Antioxidant Enzymes and Positive Muscle Work in Chronically Loaded Muscles of Aged Rats. Experimental Gerontology. 2010;45(11): 882-895.

Jaggi, S. Yadav, AS. Increased Serum Malondialdehyde Levels Among Cigarette Smokers. The Pharma Innovation Journal. 2015; 4(4):94-96.

Zorawar, S. Indrakaran, P. Karthigesu. Pramjit, S. Rupinder, K. Use of Malondialdehyde as a Biomarker for Assesing Oxidative Stress in Different Disease Pathologies: a Review. Iranian Journal Public Health. 2014; 43.(3): 7-16.

Idris, R. Hartamto, H. Pengaruh Asap Rokok Kretek Terhadap Imunitas Seluler Tikus Betina Strain LMR. Jurnal Keperawatan Indonesia. 2006;10(2); 41-47.

Rahmawati, RSN. Saputri, IK. Ratnawati, R. Soeharto, S. Wiyasa, WA. The Effect of α-Tocopherol on Oxidative Stress and Ovarian Function in Rats Exposed to Tobacco Smokes. Cukurova Medical Journal. 2014; 39 (2): 203-212.

Duran, M. Aydin, K. Oner, G. Nermin, K. Sarac, GN. Turhan, N. Possible Protective Effect of Applying Citamin E to the Rats Having Been Exposed to Cigarette Smoke: An Animal Study. Global Advanced Research Journals. 2015; 4(4):183-189.

How to Cite
Intan, W. (2020). Effect of Vitamin E Against Levels Malondyaldehyde (Mda) and Estrogen Hormone Levels Rats (Rattus Norvegicus) Females Who Exposed to Cigarette Smoke. Journal of Midwifery and Nursing, 2(2, April), 279-283. Retrieved from https://iocscience.org/ejournal/index.php/JMN/article/view/720

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.