Utilization ethanol extract of sappan wood as an alternative in escherichia coli staining
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Published:
Jan 30, 2024
Keywords:
Escherichia coli, Gram- Stainning, Mordant, ;Sappan Wood, Safranin
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Abstract
In life, sappan wood is used as an ingredient in traditional drinks that has antioxidant, antimicrobial and has stable pigments. The content of red pigment, brazilin in sappan wood can be used as a dye. The purpose of this study was to determine the potential of sappan wood ethanol extract as an alternative to safranin in gram staining. Ethanol extract of sappan wood 6% was added with alum mordant to obtain a red pigment. The bacteria used Eschericia col, isolated from water. Staining using ethanol extract of sappan wood was carried out for 30 seconds, 3, 5 and 10 minutes. This stain was compared with safranin. The results showed that staining using sappan wood extract shows clarity of the visual field, shape and good color. Based on the results, the ethanol extract of sappan wood with mordant can be used as an alternative to safranin in the gram-staining.
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How to Cite
mamay, mamay. (2024). Utilization ethanol extract of sappan wood as an alternative in escherichia coli staining. Journal of Midwifery and Nursing, 6(1), 356-360. https://doi.org/10.35335/jmn.v6i1.4565
References
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Prayekti, E. and Sumarsono, T. (2021) ‘Variations in the incubation time of the Staphylococcus aureus, Bacillus sp and Escherichia coli cultures on the results of the gram stain visualization’, IOP Conference Series: Earth and Environmental Science, 819(1), pp. 1–6. doi: 10.1088/1755-1315/819/1/012075.
Rahul et al. (2019) ‘Eco-friendly dye sensitized solar cell using natural dye with solid polymer electrolyte as hole transport material’, Materials Today: Proceedings. Elsevier Ltd., 34(xxxx), pp. 760–766. doi: 10.1016/j.matpr.2020.04.775.
Risandiansyah, R. et al. (2020) ‘The Use of Food Coloring Dyes in Bacterial Staining’, The Journal of Experimental Life Sciences, 10(2), pp. 138–143. doi: 10.21776/ub.jels.2020.010.02.10.
Singh, T. et al. (2023) ‘Natural bio-colorant and pigments: Sources and applications in food processing’, Journal of Agriculture and Food Research. Elsevier B.V., 12(May), p. 100628. doi: 10.1016/j.jafr.2023.100628.
Sofiah, S. et al. (2023) The Addition of Natural Colour Pigment Brazilin from Sappan Wood Extract (Caesalpinia Sappan Linn) in the Manufacture of Tempeh to Improve Tempeh Product Antioxidant Content. Atlantis Press International BV. doi: 10.2991/978-94-6463-118-0_6.
Vijayakumar, T. et al. (2023) ‘Diagnostic Utility of Gram Stain for Oral Smears – A Review’, Journal of Microscopy and Ultrastructure, 11(3), pp. 130–134. doi: 10.4103/jmau.jmau_108_22.
Virgianti, D. P. and Luciana, C. (2017) ‘Use of a combination extract of Angkak and teak leaves as a covering dye in gram staining’, Bakti Tunas Husada Health Journal, 17(1).
Yim, D. G. et al. (2019) ‘Effects of Caesalpinia sappan L. extract on the color stability, antioxidant and antimicrobial activity in cooked pork sausages during cold storage’, Lwt. Elsevier, 112(May), p. 108235. doi: 10.1016/j.lwt.2019.06.002.
Alaskar, A. A. and Hassabo, A. G. (2021) ‘Recent Use of Natural Animal Dyes in Various Field’, Journal of Textiles, Coloration and Polymer Science, 18(2), pp. 191–210. doi: 10.21608/jtcps.2021.79791.1067.
Asra, R. et al. (2021) ‘Application of Red Dragon Fruit Dyes as Staining in Histological Study’, Asian Journal of Pharmaceutical Research and Development, 9(6), pp. 48–45.
Ayele, A. et al. (2021) ‘Phycoremediation of Synthetic Dyes: An Effective and Eco-Friendly Algal Technology for the Dye Abatement’, Journal of Chemistry, 2021. doi: 10.1155/2021/9923643.
Bose, S. et al. (2022) ‘Histopathological Correlative Assessment of Cytoplasmic Staining By Aqueous Extracts of Hibiscus and Turmeric With Eosin on 70 Tissue Samples’, International journal of health sciences, 6(April), pp. 3915–3925. doi: 10.53730/ijhs.v6ns3.6652.
Budiasih, K. S. et al. (2023) ‘Silica Modified Natural Dye of Caesalpinnia sappan l. on Cotton Fabrics’, al-Kimiya, 10(1), pp. 31–39. doi: 10.15575/ak.v10i1.24578.
Franco-Duarte, R. et al. (2019) ‘Advances in chemical and biological methods to identify microorganisms—from past to present’, Microorganisms, 7(5). doi: 10.3390/microorganisms7050130.
Girdthep, S. et al. (2018) ‘Physico-chemical characterization of natural lake pigments obtained from Caesalpinia Sappan Linn. and their composite films for poly(lactic acid)-based packaging materials’, Dyes and Pigments. Elsevier Ltd, 157, pp. 27–39. doi: 10.1016/j.dyepig.2018.04.043.
Handayani, S. et al. (2022) ‘Synergistic Cytotoxic and Antimigratory Effect of Brazilein and Doxorubicin on HER2-Overexpressing Cells’, Asian Pacific Journal of Cancer Prevention, 23(8), pp. 2623–2632. doi: 10.31557/APJCP.2022.23.8.2623.
Hardiansyah, M. Y., Musa, Y. and Jaya, A. M. (2020) ‘Identification of Plant Growth Promoting Rhizobacteria from Thorny Bamboo Rhizosphere with 3 % KOH Gram Test and Gram Staining Test’, International Journal of Applied Biology, pp. 7–17.
Hazmi, F. Al and Oetopo, A. (2022) ‘Utilization Of Rhizophora Stylosa Bark For Natural Dyeing On Cotton Batik Fabric’, Corak, 11(1), pp. 55–56. doi: 10.24821/corak.v11i1.5727.
Heryanto, A. et al. (2023) ‘Utilization of Red Dragon Fruit Peel Natural Dye in Sperm Examination in Clinical Laboratory’, Jurnal Ilmu Kesehatan Prima Insan Cendikia, 2(2), pp. 131–136. doi: https://doi.org/10.33482/jpic.v2i2.33.
Lellis, B. et al. (2019) ‘Effects of textile dyes on health and the environment and bioremediation potential of living organisms’, Biotechnology Research and Innovation, 3(2), pp. 275–290. doi: 10.1016/j.biori.2019.09.001.
Mamay, M., Ernawati, E. and Nurisani, A. (2023) ‘Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining’, Indonesian Journal of Medical Laboratory Science and Technology, 5(2), pp. 100–111. doi: 10.33086/ijmlst.v5i2.3961.
Nathan, V. K. and Rani, M. E. (2021) ‘Natural dye from Caesalpinia sappan L. heartwood for eco-friendly coloring of recycled paper based packing material and its in silico toxicity analysis’, Environmental Science and Pollution Research. Environmental Science and Pollution Research, 28(22), pp. 28713–28719. doi: 10.1007/s11356-020-11827-4.
Ngamwonglumlert, L., Devahastin, S. and Chiewchan, N. (2020) ‘Color and molecular structure alterations of brazilein extracted from Caesalpinia sappan L . under different pH and heating conditions’, Scientific Reports. Nature Publishing Group UK, (0123456789), pp. 1–10. doi: 10.1038/s41598-020-69189-3.
Novelia A, S. D. and Asra, R. (2020) ‘Asian Journal of Pharmaceutical Research and Development A Review: Natural Resources as Natural Dyes in Pharmaceutical Products’, Asian Journal of Pharmaceutical Research and Development, 8(5), pp. 82–88. Available at: http://ajprd.com.
Nunki, N., Titik Mutiarawati, D. and Prayekti, E. (2020) ‘Purple Sweet Potato (Ipomoea batatas L.) Peels Extract as An Alternative Dye for Bacteria Gram Staining’, Indonesian Journal of Medical Laboratory Science and Technology, 2(2), pp. 76–84. doi: 10.33086/ijmlst.v2i2.1655.
Okolie, N., Aa, N. and Ib, E. (2021) ‘Staining Potentials of Indigenous Hibiscus sabdariffa Calyx, Lawsonia inermis Leaves and Vitex Doniana Stem Barks on Liver and Kidney Tissues’, Cytology & Histology Research Article, 12, p. 2021.
Pratama, M. I. et al. (2023) Potential of Methanol Extract From Rosella (Hibiscus Sabdariffa) as an Alternative Dye in Gram Staining. Atlantis Press International BV. doi: 10.2991/978-94-6463-180-7_32.
Prayekti, E. and Sumarsono, T. (2021) ‘Variations in the incubation time of the Staphylococcus aureus, Bacillus sp and Escherichia coli cultures on the results of the gram stain visualization’, IOP Conference Series: Earth and Environmental Science, 819(1), pp. 1–6. doi: 10.1088/1755-1315/819/1/012075.
Rahul et al. (2019) ‘Eco-friendly dye sensitized solar cell using natural dye with solid polymer electrolyte as hole transport material’, Materials Today: Proceedings. Elsevier Ltd., 34(xxxx), pp. 760–766. doi: 10.1016/j.matpr.2020.04.775.
Risandiansyah, R. et al. (2020) ‘The Use of Food Coloring Dyes in Bacterial Staining’, The Journal of Experimental Life Sciences, 10(2), pp. 138–143. doi: 10.21776/ub.jels.2020.010.02.10.
Singh, T. et al. (2023) ‘Natural bio-colorant and pigments: Sources and applications in food processing’, Journal of Agriculture and Food Research. Elsevier B.V., 12(May), p. 100628. doi: 10.1016/j.jafr.2023.100628.
Sofiah, S. et al. (2023) The Addition of Natural Colour Pigment Brazilin from Sappan Wood Extract (Caesalpinia Sappan Linn) in the Manufacture of Tempeh to Improve Tempeh Product Antioxidant Content. Atlantis Press International BV. doi: 10.2991/978-94-6463-118-0_6.
Vijayakumar, T. et al. (2023) ‘Diagnostic Utility of Gram Stain for Oral Smears – A Review’, Journal of Microscopy and Ultrastructure, 11(3), pp. 130–134. doi: 10.4103/jmau.jmau_108_22.
Virgianti, D. P. and Luciana, C. (2017) ‘Use of a combination extract of Angkak and teak leaves as a covering dye in gram staining’, Bakti Tunas Husada Health Journal, 17(1).
Yim, D. G. et al. (2019) ‘Effects of Caesalpinia sappan L. extract on the color stability, antioxidant and antimicrobial activity in cooked pork sausages during cold storage’, Lwt. Elsevier, 112(May), p. 108235. doi: 10.1016/j.lwt.2019.06.002.
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